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江汉大学医学与生命科学学院病理学与病理生理学教研室
内容提要
本书介绍组织病理学常用的多种技术。包括常见的技术，即福尔马林固定、石蜡包埋、HE染色技术，同时也介绍了常用的特殊染色技术，免疫组织化学技术，并简明地介绍了分子生物学技术的基本理论以及在组织病理学中的应用。
全书理论与实践并重，经典与改良结合，内容丰富，通俗易懂，实用性强，为实验室工作的必备参考书。适合从事病理学、组织学、生物学、法医学等的科研教学和临床工作的技术人员、教师、医师和研究生使用。同时也适合临床医学检验专业中专、专科生使用以及临床医学专业专升本学生和临床医学专业本科生选用。
第一篇、石蜡切片技术
第一章、组织标本的处理
- 第一节 取材·····································5
第二节 组织的固定··································6
第三节 大体标本的处理和固定·····························14
第四节 陈列标本的固定································15
第五节 脱钙·····································16
第六节 组织的冲洗··································18
第二章、石蜡包埋技术
- 第一节 脱水·····································19
第二节 透明·····································21
第三节 浸蜡·····································22
第四节 包埋·····································22
第三章 切片技术
第一节 切片刀····································25
第二节 切片机····································27
第三节 石蜡切片的制作································27
第四节 特殊组织石蜡切片的制作····························29
第五节 石蜡切片的异常及处理·····························29
第四章 染色与染色剂
第一节 染色的基本原理································31
第二节 染色剂染色的化学基础·····························33
第三节 染色剂的分类·································34
第四节 常用染色剂及配制·······························35
第五节 苏木素—伊红染色法······························39
第六节 封固剂····································41
第五章 特殊染色技术
第一节 结缔组织染色法································43
第二节 脂类染色法··································47
第三节 糖原及粘液染色法·······························48
第四节 色素染色法··································50
第五节 神经组织染色法································52
第六节 核酸及核蛋白染色法······························52
第七节 肌肉组织染色法································53
第八节 病原微生物染色法·······························54
第九节 特殊染色技术的应用······························57
第二篇 免疫组织化学和亲和免疫组织化学技术
- 第一章 免疫组织化学技术
第一节 基本原理···································62
第二节 染色步骤···································67
第三节 常用试剂的配制································72
第二章 亲和免疫组织化学技术
第一节 基本原理···································77
第二节 染色步骤···································80
第三篇 分子生物学技术
- 第一章 核酸原位杂交技术
第一节 核酸原位杂交的基本原理····························83
第二节 核酸原位杂交的主要过程····························84
第三节 核酸原位杂交的基本操作步骤··························86
第四节 常用试剂的配制································87
第五节 核酸原位杂交的应用······························89
第二章 原位PCR技术
第一节 基本原理···································91
第二节 基本类型···································91
第三节 基本步骤···································92
第四节 原位PCR技术的应用······························93
